ATM (phospho Ser1981) antibody

Cat. No. GTX132146

Reference 12

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all ATM products

View product citations for antibody GTX132146 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

351 kDa. ( Note )

Positive Control

HCT116 (UVC 100J/m2 recover 6hr)

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1981 of human ATM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886576

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus , Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 472 ATM
UniProt: Q13315 ATM

Research Area

DATA IMAGES

GTX132146 IHC-P Image

ATM (phospho Ser1981) antibody detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX132146 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:1000.

GTX132146 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132146 ICC/IF Image

ATM (phospho Ser1981) antibody detects ATM (phospho Ser1981) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Scale bar= 10 μm.

GTX132146 ICC/IF Image

GTX132146 IHC-P Image

ATM (phospho Ser1981) antibody detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody (GTX132146) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min