WB analysis of H2O2 treated (lane 2) and untreated (lane 1) MCF-7 whole cell lysate using GTX48650 ATM (phospho Ser1981) antibody.
Loading : 35 μg
Dilution : 1:1000

Western Blot of Sheep Anti-ATM pS1981 polyclonal antibody. Lane 1: untreated MCF-7 cell lysate. Lane 2: Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate. Load: 35 μg per lane. Primary antibody: ATM pS1981 antibody at 1:1000 for 1 h at room temperature. Secondary antibody: IRDye™800 conjugated Donkey anti-Sheep IgG secondary antibody at 1:5,000 for 1h at room temperature. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: 370 kDa, ATM (370 kDa) is indicated by an arrow. Other band(s): ATM splice variants and isoforms.