Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATOH7 antibody (GTX129391) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATOH7 antibody detects ATOH7 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ATOH7 antibody (GTX129391) dilution: 1:2500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ATOH7 antibody detects ATOH7 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ATOH7 antibody (GTX129391) dilution: 1:2500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ATOH7 antibody detects ATOH7 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ATOH7 antibody (GTX129391) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min