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ATOH7 antibody

Anti-ATOH7 antibody used in IHC (Paraffin sections) (IHC-P). GTX129391
Anti-ATOH7 antibody used in IHC (Paraffin sections) (IHC-P). GTX129391
Anti-ATOH7 antibody used in Western Blot (WB). GTX129391
Anti-ATOH7 antibody used in IHC (Paraffin sections) (IHC-P). GTX129391

Cat No. GTX129391

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:2500
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

ATOH7-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATOH7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

atonal bHLH transcription factor 7 , Math5 , NCRNA , PHPVAR , RNANC , bHLHa13

Cellular Localization

Nucleus

Background

ATOH7 is a member of the family of basic helix-loop-helix (bHLH) proteins with similarity to Drosophila 'atonal,' a proneural bHLH gene that controls photoreceptor development (Brown et al., 2002 [PubMed 11889557]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ATOH7 antibody used in IHC (Paraffin sections) (IHC-P). GTX129391

GTX129391 IHC-P Image

ATOH7 antibody detects ATOH7 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ATOH7 antibody (GTX129391) dilution: 1:2500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATOH7 antibody used in IHC (Paraffin sections) (IHC-P). GTX129391

GTX129391 IHC-P Image

ATOH7 antibody detects ATOH7 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ATOH7 antibody (GTX129391) dilution: 1:2500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATOH7 antibody used in Western Blot (WB). GTX129391

GTX129391 WB Image

ATOH7 antibody detects ATOH7 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human ATOH7-transfected 293T cells
15 % SDS-PAGE
ATOH7 antibody (GTX129391) dilution: 1:10000

Anti-ATOH7 antibody used in IHC (Paraffin sections) (IHC-P). GTX129391

GTX129391 IHC-P Image

ATOH7 antibody detects ATOH7 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ATOH7 antibody (GTX129391) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf