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ATP Colorimetric/Fluorometric Assay Kit

ATP Colorimetric/Fluorometric Assay Kit. GTX85579

Cat No. GTX85579

Package
100 assay ($559)
PRODUCT

Summary

ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation in the mitochondria. There are a number of commercially available ATP assays which detects femtomoles or less of ATP by measuring luminescence but these kits require specialized luminescence instrumentation and utilize luciferase which can be difficult to maintain in active form. GeneTex newly developed ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (λmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect as low as 50 picomol (1 μM) of ATP in various samples. The kit provides sufficient reagents for 100 assays.

‧ Detection method- Colorimeter (λmax = 570 nm) or fluorometer (Ex/Em = 535/587 nm)
‧ Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
‧ Species reactivity- Mammalian
‧ Kit size- 100 assays
‧ Applications- The assay can detect as low as 50 picomol (1 μM) of ATP in various samples.

Features and Benefits
‧ Simple procedure; takes less than 1 hour
‧ Fast and convenient
‧ Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.

Kit Contents:
ATP Assay Buffer
ATP Probe (lyophilized)
Dimethylsulfoxide (DMSO, Anhydrous)
ATP Converter
Developer Mix (lyophilized)
ATP Standard (1 μmol; lyophilized)

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Adenosine Triphosphate Assay , ATP Assay , ATP quantification assay

DATA IMAGES

ATP Colorimetric/Fluorometric Assay Kit. GTX85579

GTX85579 Image

Quantification of ATP concentration using fluorometric (top) or colorimetric (bottom) assay kit

REFERENCE

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Package List Price ($)
$ 559