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ATP citrate lyase antibody [3D9-E9-H8]

Anti-ATP citrate lyase antibody [3D9-E9-H8] used in Western Blot (WB). GTX49218
Anti-ATP citrate lyase antibody [3D9-E9-H8] used in Flow cytometry (FACS). GTX49218
Anti-ATP citrate lyase antibody [3D9-E9-H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49218

Cat. No. GTX49218

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3D9-E9-H8

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μg ($399)
See all ATP citrate lyase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP citrate lyase , ACL , ATPCL , CLATP

Cellular Localization

Cytoplasm

Background

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-ATP citrate lyase antibody [3D9-E9-H8] used in Western Blot (WB). GTX49218

GTX49218 WB Image

WB analysis of K562 and HeLa lysates using ATP-Citrate Lyase antibody at a dilution of 1:1,000.

Anti-ATP citrate lyase antibody [3D9-E9-H8] used in Flow cytometry (FACS). GTX49218

GTX49218 FACS Image

FACS analysis of HeLa cells using ATP-Citrate Lyase antibody at a dilution of 1:100 (red) or an isotype control (black).

Anti-ATP citrate lyase antibody [3D9-E9-H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49218

GTX49218 ICC/IF Image

ICC/IF analysis of HeLa cells using ATP-Citrate Lyase antibody at a dilution of 1:150.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399