APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , PC-12 , Rat2 , HeLa , Raji
Predict Reactivity
Sheep, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human ATP citrate lyase. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949566
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATP citrate lyase , ACL , ATPCL , CLATP
Cellular Localization
Cytoplasm
Background
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379](/templates/default/js/lightbox/images/loading.gif "Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379")
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GTX112379 WB Image
Sample (30 μg of whole cell lysate)
A: HeLa
B: Raji
7.5% SDS PAGE
GTX112379 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112379 ICC/IF Image
ATP citrate lyase antibody [C2C3], C-term detects ATP citrate lyase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATP citrate lyase protein stained by ATP citrate lyase antibody [C2C3], C-term (GTX112379) diluted at 1:200.
Blue: Hoechst 33342 staining.
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GTX112379 WB Image
Sample (50 μg of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX112379 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112379 WB Image
ATP citrate lyase antibody [C2C3], C-term detects ACLY protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
ATP citrate lyase antibody [C2C3], C-term (GTX112379) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for ATP citrate lyase antibody [C2C3], C-term (GTX112379). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ATP citrate lyase antibody [C2C3], C-term (GTX112379). Be the first to share your experience with this product.
![Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379](/upload/website/prouct_img/small/GTX112379/GTX112379_WB_w_23060500_931.webp "Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379")
![Anti-ATP citrate lyase antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112379](/upload/website/prouct_img/small/GTX112379/GTX112379_40114_20160525_IFA_w_23060500_525.webp "Anti-ATP citrate lyase antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112379")
![Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379](/upload/website/prouct_img/small/GTX112379/GTX112379_40114_WB_M_brain_w_23060500_348.webp "Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379")
![Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379](/upload/website/prouct_img/small/GTX112379/GTX112379_40114_WB_R_w_23060500_244.webp "Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379")
![Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379](/upload/website/prouct_img/normal/GTX112379/GTX112379_WB_w_23060500_931.webp "Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379")
![Anti-ATP citrate lyase antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112379](/upload/website/prouct_img/normal/GTX112379/GTX112379_40114_20160525_IFA_w_23060500_525.webp "Anti-ATP citrate lyase antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112379")
![Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379](/upload/website/prouct_img/normal/GTX112379/GTX112379_40114_WB_M_brain_w_23060500_348.webp "Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379")
![Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379](/upload/website/prouct_img/normal/GTX112379/GTX112379_40114_WB_R_w_23060500_244.webp "Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379")