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ATP citrate lyase antibody [C2C3], C-term

Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379
Anti-ATP citrate lyase antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112379
Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379
Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379

Cat. No. GTX112379

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

Positive Control

Mouse brain , PC-12 , Rat2 , HeLa , Raji

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATP citrate lyase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949566

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP citrate lyase , ACL , ATPCL , CLATP

Cellular Localization

Cytoplasm

Background

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379

GTX112379 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
B: Raji
7.5% SDS PAGE
GTX112379 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP citrate lyase antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112379

GTX112379 ICC/IF Image

ATP citrate lyase antibody [C2C3], C-term detects ATP citrate lyase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATP citrate lyase protein stained by ATP citrate lyase antibody [C2C3], C-term (GTX112379) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379

GTX112379 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX112379 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP citrate lyase antibody [C2C3], C-term used in Western Blot (WB). GTX112379

GTX112379 WB Image

ATP citrate lyase antibody [C2C3], C-term detects ACLY protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
ATP citrate lyase antibody [C2C3], C-term (GTX112379) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169