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ATP citrate lyase antibody [N1N2], N-term

Anti-ATP citrate lyase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112387
Anti-ATP citrate lyase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112387
Anti-ATP citrate lyase antibody [N1N2], N-term used in Western Blot (WB). GTX112387
Anti-ATP citrate lyase antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112387
Anti-ATP citrate lyase antibody [N1N2], N-term used in Western Blot (WB). GTX112387

Cat. No. GTX112387

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ATP citrate lyase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Sheep, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ATP citrate lyase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167814

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP citrate lyase , ACL , ATPCL , CLATP

Cellular Localization

Cytoplasm

Background

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATP citrate lyase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112387

GTX112387 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATP citrate lyase(GTX112387) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATP citrate lyase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112387

GTX112387 IHC-P Image

ATP citrate lyase antibody [N1N2], N-term detects ATP citrate lyase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
ATP citrate lyase stained by ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATP citrate lyase antibody [N1N2], N-term used in Western Blot (WB). GTX112387

GTX112387 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP citrate lyase antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112387

GTX112387 ICC/IF Image

ATP citrate lyase antibody [N1N2], N-term detects ATP citrate lyase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATP citrate lyase protein stained by ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ATP citrate lyase antibody [N1N2], N-term used in Western Blot (WB). GTX112387

GTX112387 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Garc?a-Arroyo FE et al. Biomolecules 2019; 9 (10) Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats.
Li X et al. Mol Cell 2017; 66 (5) : 684-697.e9 Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169