APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
IMR32
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.51 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATP synthase B1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10721510
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATP synthase peripheral stalk-membrane subunit b , ATP5F1 , PIG47
Cellular Localization
Mitochondrion , Mitochondrion inner membrane
Background
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX114970 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ATP synthase B1(GTX114970) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX114970 ICC/IF Image
ATP synthase B1 antibody [N1C3] detects ATP synthase B1 protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: ATP synthase B1 protein stained by ATP synthase B1 antibody [N1C3] (GTX114970) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX114970 WB Image
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE GTX114970 diluted at 1:5000
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REFERENCE
There are currently no references for ATP synthase B1 antibody [N1C3] (GTX114970). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ATP synthase B1 antibody [N1C3] (GTX114970). Be the first to share your experience with this product.