GeneTex
United States (US)

ATP synthase B1 antibody

GTX102980_WB_19011506_290.jpg
GTX102980_40135_IHC_19011506_580.jpg
GTX102980_40135_WB_M_brain_19011506_754.jpg
GTX102980_40135_IFA_19011506_134.jpg

Cat No. GTX102980

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Molt-4 , mouse brain

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP synthase B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atp Synthase Peripheral Stalk-Membrane Subunit B,Atp5F1,Pig47,Atp5Pb

Cellular Localization

Mitochondrion , Mitochondrion inner membrane

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102980_WB_19011506_290.jpg

GTX102980 WB Image

Sample (30 µg of whole cell lysate)
A: Molt-4 (GTX27912)
12% SDS PAGE
GTX102980 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102980_40135_IHC_19011506_580.jpg

GTX102980 IHC-P Image

ATP synthase B1 antibody detects ATP5F1 protein at cytosol on human colon by immunohistochemical analysis.
Sample: Paraffin-embedded colon.
ATP synthase B1 antibody (GTX102980) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102980_40135_WB_M_brain_19011506_754.jpg

GTX102980 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX102980 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102980_40135_IFA_19011506_134.jpg

GTX102980 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ATP synthase B1(GTX102980) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169