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ATP11B antibody

Anti-ATP11B antibody used in Western Blot (WB). GTX85003
Anti-ATP11B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85003
Anti-ATP11B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85003

Cat. No. GTX85003

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , K562 Cell Lysate(GTX27911)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATP11B antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP11B.The immunogen is located within amino acids 290 - 340 of ATP11B.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase phospholipid transporting 11B (putative) , ATPIF , ATPIR

Cellular Localization

Endoplasmic reticulum

Background

ATP11B is a widely expressed integral membrane ATPase and is thought to drive the transport of phospholipids across membranes. It has been suggested that ATP11B is hormonally regulated and plays a role in the subnuclear trafficking of transcription factors with RING motifs. While the exact molecule ATP11B transports is unknown, increased mRNA expression of the homologous ATP11A has been observed in cells resistant to anti-cancer drugs such as farnesyltransferase inhibitors (FTIs), suggesting that ATP11B may also play a role in cell survival under harsh conditions.

Database

Research Area

DATA IMAGES

Anti-ATP11B antibody used in Western Blot (WB). GTX85003

GTX85003 WB Image

WB analysis of K562 cell tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85003 ATP11B antibody.
Working concentration : 1 μg/ml

Anti-ATP11B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85003

GTX85003 ICC/IF Image

ICC/IF analysis of K562 cells using GTX85003 ATP11B antibody.
Working concentration : 10 μg/ml

Anti-ATP11B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85003

GTX85003 ICC/IF Image

ICC/IF analysis of K562 cells using GTX85003 ATP11B antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399