GeneTex
United States (US)

ATP5A1 antibody

GTX33028_20180530_WB_18121410_227.jpg
GTX33028_20180530_IHC-P_18121410_687.jpg
GTX33028_20180530_ICCIF_18121410_853.jpg
GTX33028_20180530_IHC-P_1_18121410_586.jpg
GTX33028_20180530_IP_18121410_365.jpg

Cat No. GTX33028

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atp Synthase F1 Subunit Alpha,Atp5A,Atp5A1,Atp5Al2,Atpm,Coxpd22,Hel-S-123M,Mc5Dn4,Mom2,Omr,Orm,Hatp1,Atp5F1A

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES
GTX33028_20180530_WB_18121410_227.jpg

GTX33028 WB Image

WB analysis of various samples using GTX33028 ATP5A1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX33028_20180530_IHC-P_18121410_687.jpg

GTX33028 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX33028 ATP5A1 antibody.
Dilution : 1:100

GTX33028_20180530_ICCIF_18121410_853.jpg

GTX33028 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX33028 ATP5A1 antibody.
Blue : DAPI

GTX33028_20180530_IHC-P_1_18121410_586.jpg

GTX33028 IHC-P Image

IHC-P analysis of human liver injury tissue using GTX33028 ATP5A1 antibody.
Dilution : 1:100

GTX33028_20180530_IP_18121410_365.jpg

GTX33028 IP Image

IP analysis of MCF-7 cell lysate using GTX33028 ATP5A1 antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

Package List Price ($)
$ 329