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ATP5B antibody [4.3E8.D10]

Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432
Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432
Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432
Anti-ATP5B antibody [4.3E8.D10] used in Western Blot (WB). GTX25432
Anti-ATP5B antibody [4.3E8.D10] used in Immunoprecipitation (IP). GTX25432
Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432

Cat No. GTX25432

Host Mouse
Clonality Monoclonal
Clone Name 4.3E8.D10
Isotype IgG1
Application WB, ICC/IF, IP, IHC
Reactivity Human, Mouse, Rat
Package
100 μg ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
ICC/IF 1:100-1:1000
IP 2-5 μg
IHC Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Intact rat mitochondria.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atp Synthase F1 Subunit Beta,Atp5B,Atp5F1B

Cellular Localization

Mitochondrion inner membrane

Background

beta subunit of rat liver ATP synthase [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432

GTX25432 ICC/IF Image

ICC/IF analysis of A431 cells using GTX25432 ATP5B antibody [4.3E8.D10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4℃

Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432

GTX25432 ICC/IF Image

ICC/IF analysis of rat neuronal/glial cell culture using GTX25432 ATP5B antibody [4.3E8.D10].

Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432

GTX25432 ICC/IF Image

ICC/IF analysis of HEK293T cells using GTX25432 ATP5B antibody [4.3E8.D10].
Red :ATPSynthase beta (ATP5B )
Blue : Nuclei
Fixation : formaldehyde
Permeabilization : 0.3% Triton X-100
Dilution : 1:100 overnight at 4 °C

Anti-ATP5B antibody [4.3E8.D10] used in Western Blot (WB). GTX25432

GTX25432 WB Image

WB analysis of rat neuronal/glial cell extract using GTX25432 ATP5B antibody [4.3E8.D10].

Anti-ATP5B antibody [4.3E8.D10] used in Immunoprecipitation (IP). GTX25432

GTX25432 IP Image

IP analysis of THP-1 cell lysates using GTX25432 ATP5B antibody [4.3E8.D10].
GTX25432 was used as IP and IB antibody
IP reaction : 5μl antibody / 500μg lysate
Dilution : 1:1000

Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432

GTX25432 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX25432 ATP5B antibody [4.3E8.D10]. Cells were probed without (left) or with(right) an antibody.
Green : ATPSynthase beta (ATP5B )
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Dilution : 5 µg/ml for 1 hour at room temperature

REFERENCE
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