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ATP5B antibody [7F4]

Overexpression
Anti-ATP5B antibody [7F4] used in Western Blot (WB). GTX84843
Anti-ATP5B antibody [7F4] used in Western Blot (WB). GTX84843
Anti-ATP5B antibody [7F4] used in Flow cytometry (FACS). GTX84843
Anti-ATP5B antibody [7F4] used in IHC (Paraffin sections) (IHC-P). GTX84843
Anti-ATP5B antibody [7F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84843
Anti-ATP5B antibody [7F4] used in IHC (Paraffin sections) (IHC-P). GTX84843
Anti-ATP5B antibody [7F4] used in IHC (Paraffin sections) (IHC-P). GTX84843
Anti-ATP5B antibody [7F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84843
Anti-ATP5B antibody [7F4] used in Flow cytometry (FACS). GTX84843

Cat. No. GTX84843

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7F4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all ATP5B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP synthase F1 subunit beta , ATP5B , ATPMB , ATPSB , HEL-S-271

Cellular Localization

Mitochondrion inner membrane

Background

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Database

  • Gene ID: 506 ATP5F1B
  • UniProt: P06576 ATP5F1B

Research Area

DATA IMAGES

Anti-ATP5B antibody [7F4] used in Western Blot (WB). GTX84843

GTX84843 WB Image

Overexpression

WB analysis of HEK293T cells transfected with ATP5B plasmid (Right) or empty vector (Left) for 48 hrs using GTX84843 ATP5B antibody [7F4].
Loading : 5 ug per lane

Anti-ATP5B antibody [7F4] used in Western Blot (WB). GTX84843

GTX84843 WB Image

WB analysis of various cell lines using GTX84843 ATP5B antibody [7F4].
Loading : 35 ug per lane

Anti-ATP5B antibody [7F4] used in Flow cytometry (FACS). GTX84843

GTX84843 FACS Image

FACS analysis of Jurkat cells using GTX84843 ATP5B antibody [7F4].
Red : Primary antibody
Blue : Negative control antibody

Anti-ATP5B antibody [7F4] used in IHC (Paraffin sections) (IHC-P). GTX84843

GTX84843 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX84843 ATP5B antibody [7F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ATP5B antibody [7F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84843

GTX84843 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84843 ATP5B antibody [7F4].

Anti-ATP5B antibody [7F4] used in IHC (Paraffin sections) (IHC-P). GTX84843

GTX84843 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84843 ATP5B antibody [7F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ATP5B antibody [7F4] used in IHC (Paraffin sections) (IHC-P). GTX84843

GTX84843 IHC-P Image

IHC-P analysis of endometrium adenocarcinoma tissue using GTX84843 ATP5B antibody [7F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ATP5B antibody [7F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84843

GTX84843 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with ATP5B plasmid using GTX84843 ATP5B antibody [7F4].

Anti-ATP5B antibody [7F4] used in Flow cytometry (FACS). GTX84843

GTX84843 FACS Image

FACS analysis of HEK293T cells transfected with either ATP5B plasmid(Red) or empty vector control plasmid(Blue) using GTX84843 ATP5B antibody [7F4].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519