APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:25 |
| 1:50-1:100 |
| 1:10-1:50 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human ATP5B.
Purification
Protein A purified, followed by peptide affinity purification.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATP synthase F1 subunit beta , ATP5B , ATPMB , ATPSB , HEL-S-271
Cellular Localization
Mitochondrion inner membrane
Background
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX81939 IHC-P Image
IHC-P analysis of human brain tissue using GTX81939 ATP5B antibody, Internal.
|
|
|
GTX81939 FACS Image
FACS analysis of WiDr cells using GTX81939 ATP5B antibody, Internal.
Green : primary antibody
Blue : negative control
|
|
|
GTX81939 IHC-P Image
IHC-P analysis of human liver tissue using GTX81939 ATP5B antibody, Internal.
Dilution : 1:25
|
|
|
GTX81939 WB Image
WB analysis of WiDr cell lysate using GTX81939 ATP5B antibody, Internal.
Lane 1 : pre-incubated without blocking peptide
Lane 2 : pre-incubate with blocking peptide
|
|
|
GTX81939 IHC-P Image
IHC-P analysis of human small intestine using GTX81939 ATP5B antibody, Internal.
Dilution : 1:25
|
|
|
GTX81939 WB Image
WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B (Lane 2) using GTX81939 ATP5B antibody, Internal.
|
|
|
GTX81939 ICC/IF Image
ICC/IF analysis of SK-Br-3 cells using GTX81939 ATP5B antibody, Internal.
Green : ATP5B
Blue : DAPI
Dilution : 1:25
|
REFERENCE
There are currently no references for ATP5B antibody, Internal (GTX81939). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ATP5B antibody, Internal (GTX81939). Be the first to share your experience with this product.
