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ATP5B antibody

Anti-ATP5B antibody used in Western Blot (WB). GTX132925
Anti-ATP5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132925

Cat No. GTX132925

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.520 mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATP5B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ATP synthase F1 subunit beta,ATP5B,ATPMB,ATPSB,HEL-S-271

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 506 ATP5F1B
  • UniProt: P06576 ATP5F1B

Research Area

DATA IMAGES
Anti-ATP5B antibody used in Western Blot (WB). GTX132925

GTX132925 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5B antibody (GTX132925) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATP5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132925

GTX132925 ICC/IF Image

ATP5B antibody detects ATP5B protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATP5B stained by ATP5B antibody (GTX132925) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

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Package List Price ($)
$ 319
$ 169