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ATP5B antibody

Anti-ATP5B antibody used in IHC (Paraffin sections) (IHC-P). GTX30075
Anti-ATP5B antibody used in Western Blot (WB). GTX30075
Anti-ATP5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30075
Anti-ATP5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30075
Anti-ATP5B antibody used in IHC (Paraffin sections) (IHC-P). GTX30075
Anti-ATP5B antibody used in IHC (Paraffin sections) (IHC-P). GTX30075
Anti-ATP5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30075
Anti-ATP5B antibody used in IHC (Paraffin sections) (IHC-P). GTX30075

Cat. No. GTX30075

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ATP5B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B (NP_001677.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP synthase F1 subunit beta , ATP5B , ATPMB , ATPSB , HEL-S-271

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 506 ATP5F1B
  • UniProt: P06576 ATP5F1B

Research Area

DATA IMAGES

Anti-ATP5B antibody used in IHC (Paraffin sections) (IHC-P). GTX30075

GTX30075 IHC-P Image

IHC-P analysis of rat heart tissue using GTX30075 ATP5B antibody.
Dilution : 1:100

Anti-ATP5B antibody used in Western Blot (WB). GTX30075

GTX30075 WB Image

WB analysis of various sample lysates using GTX30075 ATP5B antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ATP5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30075

GTX30075 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX30075 ATP5B antibody.
Blue : DAPI
Dilution : 1:100

Anti-ATP5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30075

GTX30075 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX30075 ATP5B antibody.
Blue : DAPI
Dilution : 1:100

Anti-ATP5B antibody used in IHC (Paraffin sections) (IHC-P). GTX30075

GTX30075 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX30075 ATP5B antibody.
Dilution : 1:100

Anti-ATP5B antibody used in IHC (Paraffin sections) (IHC-P). GTX30075

GTX30075 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX30075 ATP5B antibody.
Dilution : 1:100

Anti-ATP5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30075

GTX30075 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30075 ATP5B antibody.
Blue : DAPI
Dilution : 1:100

Anti-ATP5B antibody used in IHC (Paraffin sections) (IHC-P). GTX30075

GTX30075 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX30075 ATP5B antibody.
Dilution : 1:100

REFERENCE

Application Reference

Syed Nooruzuha Barmaver et al. Research Square 2023; A Sideroexin/Connexin adaptor ties kinesin-3 to mitochondria in neurons
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399