HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with ATP5D antibody (GTX101503) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101503 diluted at 1:1000

ATP synthase delta antibody detects ATP5D protein at mitochondria on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
ATP synthase delta antibody (GTX101503) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min