APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant funsion protein containing a sequence corresponding to amino acids 1-168 of human ATP5D (NP_001678.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATP synthase F1 subunit delta , ATP5D , MC5DN5
Cellular Localization
Mitochondrion,Mitochondrion inner membrane
Background
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX66588 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX66588 ATP5D antibody.
Blue : DAPI
Dilution : 1:100
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GTX66588 WB Image
WB analysis of various sample lysates using GTX66588 ATP5D antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX66588 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX66588 ATP5D antibody.
Blue : DAPI
Dilution : 1:100
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GTX66588 ICC/IF Image
ICC/IF analysis of C6 cells using GTX66588 ATP5D antibody.
Blue : DAPI
Dilution : 1:100
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REFERENCE
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REVIEW
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