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ATP5G1 antibody [N1C1]

Anti-ATP5G1 antibody [N1C1] used in Western Blot (WB). GTX104671
Anti-ATP5G1 antibody [N1C1] used in Western Blot (WB). GTX104671

Cat. No. GTX104671

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATP5G1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ATP5G1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240492

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP synthase membrane subunit c locus 1 , ATP5A , ATP5G , ATP5G1

Cellular Localization

Mitochondrion membrane

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]

Database

  • Gene ID: 516 ATP5MC1
  • UniProt: P05496 ATP5MC1

Research Area

DATA IMAGES

Anti-ATP5G1 antibody [N1C1] used in Western Blot (WB). GTX104671

GTX104671 WB Image

Sample (30 μg whole cell lysate)
A:MOLT4 (GTX27912)
15% SDS PAGE
GTX104671 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP5G1 antibody [N1C1] used in Western Blot (WB). GTX104671

GTX104671 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28701793

REFERENCE

Application Reference

Muluhngwi P et al. Sci Rep 2017; 7 (1) : 5205 The miR-29 transcriptome in endocrine-sensitive and resistant breast cancer cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169