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ATP5H antibody [6B12]

Anti-ATP5H antibody [6B12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04924
Anti-ATP5H antibody [6B12] used in Western Blot (WB). GTX04924
Anti-ATP5H antibody [6B12] used in IHC (Paraffin sections) (IHC-P). GTX04924
Anti-ATP5H antibody [6B12] used in IHC (Paraffin sections) (IHC-P). GTX04924

Cat. No. GTX04924

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6B12

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μg ($469)
See all ATP5H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 2μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

0.05mg Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human ATP5H recombinant protein (Position: A2-L161)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP synthase, H+ transporting, mitochondrial Fo complex subunit D,ATP5H,ATPQ,ATP synthase peripheral stalk subunit d

Cellular Localization

Mitochondrion,Mitochondrion inner membrane

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-ATP5H antibody [6B12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04924

GTX04924 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX04924 ATP5H antibody [6B12].
Antigen retrieval : Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent.
Red : Primary antibody
Blue : DAPI
Dilution : 2 μg/ml

Anti-ATP5H antibody [6B12] used in Western Blot (WB). GTX04924

GTX04924 WB Image

WB analysis of various samples using GTX04924 ATP5H antibody [6B12].
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : U-87MG whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : COS-7 whole cell lysate
Lane 6 : SW620 whole cell lysate
Lane 7 : PC-12 whole cell lysate
Lane 8 : Ana-1 whole cell lysate
Loading : 50μg
Dilution : 0.5 μg/mL

Anti-ATP5H antibody [6B12] used in IHC (Paraffin sections) (IHC-P). GTX04924

GTX04924 IHC-P Image

IHC-P analysis of human B lymphocytic tumor tissue using GTX04924 ATP5H antibody [6B12].
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0)
Dilution : 1 μg/ml

Anti-ATP5H antibody [6B12] used in IHC (Paraffin sections) (IHC-P). GTX04924

GTX04924 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX04924 ATP5H antibody [6B12].
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0)
Dilution : 1 μg/ml

REFERENCE

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Package List Price ($)
$ 469