GeneTex
United States (US)

ATP5H antibody

ATP5H-antibody-GTX87685-WB-1_18121409_220.jpg

Cat No. GTX87685

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Specificity/Sensitivity

ATP5H Antibody detects endogenous levels of total ATP5H protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ATP5H.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ATP5H, ATP synthase subunit d, mitochondrial

Background

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Show More +

Database

Research Area

DATA IMAGES
ATP5H-antibody-GTX87685-WB-1_18121409_220.jpg

GTX87685 WB Image

Western blot analysis of extracts from HepG2/Jurkat cells, using ATP5H Antibody. The lane on the right is treated with the synthesized peptide.

Reference