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ATP5H antibody

Anti-ATP5H antibody used in Western Blot (WB). GTX87685

Cat. No. GTX87685

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Rat
Package
100 μg ($409)
See all ATP5H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ATP5H (111-160).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10731034

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATP synthase, H+ transporting, mitochondrial Fo complex subunit D , ATP5H , ATPQ

Cellular Localization

Mitochondrion,Mitochondrion inner membrane

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-ATP5H antibody used in Western Blot (WB). GTX87685

GTX87685 WB Image

WB analysis of HepG2 and Jurkat cell lysates using GTX87685 ATP5H antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Gerard N et al. Mol Nutr Food Res 2014; Proteomic analysis identifies cytoskeleton-interacting proteins as major downstream targets of altered folate status in the aorta of adult rat.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409