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ATP5J antibody

Anti-ATP5J antibody used in Western Blot (WB). GTX110996
Anti-ATP5J antibody used in IHC (Paraffin sections) (IHC-P). GTX110996
Anti-ATP5J antibody used in Western Blot (WB). GTX110996
Anti-ATP5J antibody used in Western Blot (WB). GTX110996
Anti-ATP5J antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110996

Cat. No. GTX110996

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ATP5J products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

HeLa membrane

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ATP5J Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162595

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP synthase peripheral stalk subunit F6 , ATP5 , ATP5A , ATP5J , ATPM , CF6 , F6

Cellular Localization

Mitochondrion , Mitochondrion inner membrane

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the F0 complex, required for F1 and F0 interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATP5J antibody used in Western Blot (WB). GTX110996

GTX110996 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody (GTX110996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP5J antibody used in IHC (Paraffin sections) (IHC-P). GTX110996

GTX110996 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using ATP5J(GTX110996) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATP5J antibody used in Western Blot (WB). GTX110996

GTX110996 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody (GTX110996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP5J antibody used in Western Blot (WB). GTX110996

GTX110996 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HeLa membrane
15% SDS PAGE
GTX110996 diluted at 1:1000

Anti-ATP5J antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110996

GTX110996 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ATP5J(GTX110996) antibody at 1:500 dilution.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169