Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody (GTX110996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody (GTX110996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody (GTX110996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using ATP5J(GTX110996) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: HeLa
B: HeLa membrane
15% SDS PAGE
GTX110996 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using ATP5J(GTX110996) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min