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ATP6V1A antibody [GT1561]

KO/KD
Anti-ATP6V1A antibody [GT1561] used in Western Blot (WB). GTX633544
Anti-ATP6V1A antibody [GT1561] used in Western Blot (WB). GTX633544
Anti-ATP6V1A antibody [GT1561] used in Flow cytometry (FACS). GTX633544

Cat. No. GTX633544

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1561

Isotype

 IgG1

Application

 WB, FACS

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATP6V1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

HepG2 , 293T

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888347

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase H+ transporting V1 subunit A , ARCL2D , ATP6A1 , ATP6V1A1 , HO68 , IECEE3 , VA68 , VPP2 , Vma1

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Database

  • Gene ID: 523 ATP6V1A
  • UniProt: P38606 ATP6V1A

Research Area

DATA IMAGES

Anti-ATP6V1A antibody [GT1561] used in Western Blot (WB). GTX633544

GTX633544 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT1561] (GTX633544) diluted at 1:500.

Anti-ATP6V1A antibody [GT1561] used in Western Blot (WB). GTX633544

GTX633544 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT1561] (GTX633544) diluted at 1:1000.

Anti-ATP6V1A antibody [GT1561] used in Flow cytometry (FACS). GTX633544

GTX633544 FACS Image

ATP6V1A antibody [GT1561] (GTX633544) detects ATP6V1A protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: ATP6V1A antibody [GT1561] (GTX633544) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations