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ATP6V1A antibody

Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815
Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815
Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815
Anti-ATP6V1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110815
Anti-ATP6V1A antibody used in Immunoprecipitation (IP). GTX110815
Anti-ATP6V1A antibody used in IHC (Paraffin sections) (IHC-P). GTX110815

Cat No. GTX110815

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATPase H+ transporting V1 subunit A , ARCL2D , ATP6A1 , ATP6V1A1 , HO68 , IECEE3 , VA68 , VPP2 , Vma1

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Database

  • Gene ID: 523 ATP6V1A
  • UniProt: P38606 ATP6V1A

Research Area

DATA IMAGES

Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815

GTX110815 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody (GTX110815) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815

GTX110815 WB Image

ATP6V1A antibody detects ATP6V1A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody (GTX110815) diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815

GTX110815 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX110815 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP6V1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110815

GTX110815 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using ATP6V1A(GTX110815) antibody at 1:500 dilution.

Anti-ATP6V1A antibody used in Immunoprecipitation (IP). GTX110815

GTX110815 IP Image

Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 μg of ATP6V1A antibody (GTX110815).
Western blot analysis was performed using ATP6V1A antibody (GTX110815).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ATP6V1A antibody used in IHC (Paraffin sections) (IHC-P). GTX110815

GTX110815 IHC-P Image

ATP6V1A antibody detects ATP6V1A protein at cytoplasm on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ATP6V1A antibody (GTX110815) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169