APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain
Predict Reactivity
Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATP6V1B2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885290
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATPase H+ transporting V1 subunit B2 , ATP6B1B2 , ATP6B2 , DOOD , HO57 , VATB , VPP3 , Vma2 , ZLS2
Cellular Localization
Endomembrane system; Peripheral membrane protein , Melanosome
Background
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]
Database
-
Gene ID: 526
ATP6V1B2
-
UniProt: P21281
ATP6V1B2
Research Area
DATA IMAGES
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GTX110783 WB Image
ATP6V1B2 antibody detects ATP6V1B2 protein by Western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
7.5 % SDS-PAGE
ATP6V1B2 antibody (GTX110783) dilution: 1:1000
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GTX110783 IHC-P Image
ATP6V1B2 antibody detects ATP6V1B2 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ATP6V1B2 antibody (GTX110783) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110783 WB Image
ATP6V1B2 antibody detects ATP6V1B2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5 % SDS-PAGE
ATP6V1B2 antibody (GTX110783) dilution: 1:5000
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GTX110783 WB Image
ATP6V1B2 antibody detects ATP6V1B2 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5 % SDS-PAGE
ATP6V1B2 antibody (GTX110783) dilution: 1:5000
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GTX110783 WB Image
The data was published in the journal Cancers (Basel) in 2019.PMID: 31717697
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GTX110783 WB Image
The data was published in the journal Cancers (Basel) in 2019.PMID: 31717697
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GTX110783 WB Image
The data was published in the journal Cancers (Basel) in 2019.PMID: 31717697
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REFERENCE
REVIEW
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