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ATP6V1B2 antibody

Anti-ATP6V1B2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32461
Anti-ATP6V1B2 antibody used in Western Blot (WB). GTX32461

Cat No. GTX32461

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
50 μl ($299)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 262-511 of human ATP6V1B2 (NP_001684.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATPase H+ transporting V1 subunit B2 , ATP6B1B2 , ATP6B2 , DOOD , HO57 , VATB , VPP3 , Vma2 , ZLS2

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 526 ATP6V1B2
  • UniProt: P21281 ATP6V1B2

Research Area

DATA IMAGES

Anti-ATP6V1B2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32461

GTX32461 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32461 ATP6V1B2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ATP6V1B2 antibody used in Western Blot (WB). GTX32461

GTX32461 WB Image

WB analysis of various sample lysates using GTX32461 ATP6V1B2 antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 299