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ATP6V1E2 antibody [N1C3]

Anti-ATP6V1E2 antibody [N1C3] used in Western Blot (WB). GTX111015
Anti-ATP6V1E2 antibody [N1C3] used in Western Blot (WB). GTX111015

Cat. No. GTX111015

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all ATP6V1E2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Human, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V1E2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171719

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase H+ transporting V1 subunit E2 , ATP6E1 , ATP6EL2 , ATP6V1EL2 , VMA4

Background

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome.

Database

Research Area

DATA IMAGES

Anti-ATP6V1E2 antibody [N1C3] used in Western Blot (WB). GTX111015

GTX111015 WB Image

Western blot experiment using ATP6V1E2 Antibody (GTX111015) at 1:90000 dilution to detect 100ng of 34kDa recombinant ATP6V1E2 protein antigen.

Anti-ATP6V1E2 antibody [N1C3] used in Western Blot (WB). GTX111015

GTX111015 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX111015 diluted at 1:10000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169