ATP6V1H antibody [N3C3]

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

ATP6V1H antibody [N3C3] detects ATP6V1H protein by immunofluorescent analysis.
Sample: T-47D cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATP6V1H stained by ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

ATP6V1H antibody [N3C3] detects ATP6V1H protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ATP6V1H stained by ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ATP6V1H antibody [N3C3] detects ATP6V1H protein at cytoplasm by immunofluorescent analysis.
Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATP6V1H stained by ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:500.
Red: Tau, a marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

ATP6V1H antibody [N3C3] detects ATP6V1H protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATP6V1H antibody [N3C3] detects Atp6v1h protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
ATP6V1H antibody [N3C3] (GTX110778) dilution: 1:50.

Zebrafish tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.