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ATP6V1H antibody [N3C3]

Orthogonal
Anti-ATP6V1H antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110778
Anti-ATP6V1H antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX110778
Anti-ATP6V1H antibody [N3C3] used in Western Blot (WB). GTX110778
Anti-ATP6V1H antibody [N3C3] used in Western Blot (WB). GTX110778
Anti-ATP6V1H antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX110778
Anti-ATP6V1H antibody [N3C3] used in Western Blot (WB). GTX110778
Anti-ATP6V1H antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX110778
Anti-ATP6V1H antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110778

Cat. No. GTX110778

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($419),
25 μl ($169)
See all ATP6V1H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949706

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase H+ transporting V1 subunit H , CGI-11 , MSTP042 , NBP1 , SFD , SFDalpha , SFDbeta , VMA13

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATP6V1H antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110778

GTX110778 ICC/IF Image

ATP6V1H antibody [N3C3] detects ATP6V1H protein by immunofluorescent analysis.
Sample: T-47D cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATP6V1H stained by ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ATP6V1H antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX110778

GTX110778 IHC-P Image

ATP6V1H antibody [N3C3] detects ATP6V1H protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ATP6V1H stained by ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATP6V1H antibody [N3C3] used in Western Blot (WB). GTX110778

GTX110778 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ATP6V1H antibody [N3C3] used in Western Blot (WB). GTX110778

GTX110778 WB Image

Zebrafish tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATP6V1H antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX110778

GTX110778 IHC-Wm Image

ATP6V1H antibody [N3C3] detects Atp6v1h protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
ATP6V1H antibody [N3C3] (GTX110778) dilution: 1:50.
Anti-ATP6V1H antibody [N3C3] used in Western Blot (WB). GTX110778

GTX110778 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Double bands were observed. It is possibly due to alternative splicing.
Reference:PMID: 28970149

Anti-ATP6V1H antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX110778

GTX110778 IHC-P Image

ATP6V1H antibody [N3C3] detects ATP6V1H protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATP6V1H antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110778

GTX110778 ICC/IF Image

ATP6V1H antibody [N3C3] detects ATP6V1H protein at cytoplasm by immunofluorescent analysis.
Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATP6V1H stained by ATP6V1H antibody [N3C3] (GTX110778) diluted at 1:500.
Red: Tau, a marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

REFERENCE

Application Reference

Choy CH et al. J Cell Sci 2018; (Epub) Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence.
Application : WB
Reactivity : Mouse
Hipolito V et al. bioRxiv 2018; (Epub) Lysosome expansion by selective translation of lysosomal transcripts during phagocyte activation.
Application : WB
Reactivity : Mouse
Gondim MV et al. J Virol 2015; 89 (24) : 12518-24 AP-2 Is the Crucial Clathrin Adaptor Protein for CD4 Downmodulation by HIV-1 Nef in Infected Primary CD4+ T Cells.
MARCOS VINICIUS PEREIRA GONDIM et al. 0 2014; MECANISMOS MOLECULARES DAS PROTEINAS ACESSORIAS NEF E VPU RELACIONADOS A PATOGENESE DO HIV-1
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169