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ATP7b antibody

Anti-ATP7b antibody used in Western Blot (WB). GTX04095
Anti-ATP7b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04095
Anti-ATP7b antibody used in IHC (Paraffin sections) (IHC-P). GTX04095
Anti-ATP7b antibody used in Western Blot (WB). GTX04095
Anti-ATP7b antibody used in Western Blot (WB). GTX04095
Anti-ATP7b antibody used in Western Blot (WB). GTX04095
Anti-ATP7b antibody used in Flow cytometry (FACS). GTX04095

Cat. No. GTX04095

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
400 μl ($399)
See all ATP7b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

157 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 1361-1391 amino acids from the C-terminal region of human ATP7B.

Purification

Protein A purified followed by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase copper transporting beta , PWD , WC1 , WD , WND

Background

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ATP7b antibody used in Western Blot (WB). GTX04095

GTX04095 WB Image

WB analysis of rat liver tissue lysates using GTX04095 ATP7b antibody.
Loading : 20 μg
Dilution : 1:2000

Anti-ATP7b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04095

GTX04095 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX04095 ATP7b antibody.
Green : Primary antibody
Blue : DAPI

Anti-ATP7b antibody used in IHC (Paraffin sections) (IHC-P). GTX04095

GTX04095 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX04095 ATP7b antibody.

Anti-ATP7b antibody used in Western Blot (WB). GTX04095

GTX04095 WB Image

WB analysis of various lysates using GTX04095 ATP7b antibody.
Lane 1 : Caco-2 cells
Lane 2 : Mouse kidney
Lane 3 : Rat liver
Loading : 20 μg per lane
Dilution : 1:2000

Anti-ATP7b antibody used in Western Blot (WB). GTX04095

GTX04095 WB Image

WB analysis of mouse liver tissue lysates using GTX04095 ATP7b antibody.
Loading : 20 μg
Dilution : 1:2000

Anti-ATP7b antibody used in Western Blot (WB). GTX04095

GTX04095 WB Image

WB analysis of Caco-2 cell lysates using GTX04095 ATP7b antibody.
Loading : 20 μg
Dilution : 1:2000

Anti-ATP7b antibody used in Flow cytometry (FACS). GTX04095

GTX04095 FACS Image

FACS analysis of HepG2 cells using GTX04095 ATP7b antibody.
Right histogram : Primary antibody
Left histogram : Negative comtrol

REFERENCE

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REVIEW

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Package List Price ($)
$ 399