GeneTex
United States (US)

ATP7b antibody

GTX30639_219_ICC-IF_18121410_421.jpg
GTX30639_331_IHC-P_18121410_808.jpg
GTX30639_1447_WB_18121410_490.jpg

Cat No. GTX30639

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 2 - 10 μg/ml
IHC-P 1:100 - 1:500
IP Assay dependent
IHC 1:100 - 1:500
Not tested in other applications.

Calculated MW

157 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to an internal sequence near the N-terminus of human ATP7b. [UniProt# P35670]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atpase Copper Transporting Beta,Pwd,Wc1,Wd,Wnd,Atp7B

Cellular Localization

Golgi apparatus,trans-Golgi network membrane, Multi-pass membrane protein,Isoform 2: Cytoplasm,WND/140 kDa: Mitochondrion,Golgi apparatus,trans-Golgi network membrane,Isoform 2: Cytoplasm,WND/140 kDa: Mitochondrion

Background

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30639_219_ICC-IF_18121410_421.jpg

GTX30639 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30639 ATP7b antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:100
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

GTX30639_331_IHC-P_18121410_808.jpg

GTX30639 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX30639 ATP7b antibody.

GTX30639_1447_WB_18121410_490.jpg

GTX30639 WB Image

WB analysis of mouse brain membrane fraction extract using GTX30639 ATP7b antibody.
Loading : 20μg

REFERENCE
Package List Price ($)
$ 329