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ATP7b antibody

Anti-ATP7b antibody used in Western Blot (WB). GTX30639
Anti-ATP7b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30639
Anti-ATP7b antibody used in IHC (Paraffin sections) (IHC-P). GTX30639

Cat. No. GTX30639

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC, PLA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all ATP7b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 2 - 10 μg/ml
IHC-P 1:100 - 1:500
IP Assay dependent
IHC 1:100 - 1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

157 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to an internal sequence near the N-terminus of human ATP7b. [UniProt# P35670]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_625804

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase copper transporting beta , PWD , WC1 , WD , WND

Background

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATP7b antibody used in Western Blot (WB). GTX30639

GTX30639 WB Image

WB analysis of mouse brain membrane fraction extract using GTX30639 ATP7b antibody.
Loading : 20μg

Anti-ATP7b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30639

GTX30639 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30639 ATP7b antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:100
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-ATP7b antibody used in IHC (Paraffin sections) (IHC-P). GTX30639

GTX30639 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX30639 ATP7b antibody.

REFERENCE

Application Reference

L El Nachef et al. Biomolecules 2023; 13 (12) : 1746 Response of Fibroblasts from Menkes’ and Wilson’s Copper Metabolism-Related Disorders to Ionizing Radiation: Influence of the Nucleo-Shuttling of the ATM Protein Kinase
Application : ICC/IF, PLA
Valtorta S et al. J Nucl Med 2013; 54 (7) : 1106-12 Comparison of 18F-fluoroazomycin-arabinofuranoside and 64Cu-diacetyl-bis(N4-methylthiosemicarbazone) in preclinical models of cancer.
Application : IHC
Reactivity : Mouse
Roybal JL et al. Gene Ther 2012; 19 (11) : 1085-94 Early gestational gene transfer with targeted ATP7B expression in the liver improves phenotype in a murine model of Wilson's disease.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419
Currently in production