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ATR antibody [2B5]

Orthogonal
Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109
Anti-ATR antibody [2B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70109
Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109
Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

Cat. No. GTX70109

Reference 29

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2B5

Isotype

 IgG1

Application

 WB, ICC/IF, IP, Functional Assay

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all ATR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

301 kDa. ( Note )

Positive Control

Raji , Raji nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368158

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

GTX70109 WB Image

Orthogonal

Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody (GTX70109) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATR antibody [2B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70109

GTX70109 ICC/IF Image

Immunofluorescence analysis of HeLa, using ATR(GTX70109) antibody at 1:100 dilution.

Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

GTX70109 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] (GTX70109) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

GTX70109 WB Image

Detection of ATM (Anti-ATM 2C1, GTX70103) or ATR (Anti-ATR 2B5,GTX70109) by western blot in human Raji whole cell extract.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Alzhanova D et al. PLoS Pathog 2021; 17 (1) : e1009033 Novel modulators of p53-signaling encoded by unknown genes of emerging viruses.
Application : WB
Reactivity : Human
Sun X et al. Nucleic Acids Res 2020; 48 (16) : 9109-9123 NRF2 preserves genomic integrity by facilitating ATR activation and G2 cell cycle arrest.
Yenerall P et al. Cell Chem Biol 2020; 27 (1) : 105-121.e14 RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
Application : WB
Jingwen Li et al. RSC Adv 2019; 9 (67) : 39082-39089 New spirobisnaphthalenes from an endolichenic fungus strain CGMCC 3.15192 and their anticancer effects through the P53-P21 pathway.
Application : WB
Reactivity : Human
Chen X et al. J Cancer Res Clin Oncol 2019; 145 (6) : 1471-1484 IL-6 signaling contributes to radioresistance of prostate cancer through key DNA repair-associated molecules ATM, ATR, and BRCA 1/2.
Application : ICC/IF
Reactivity : Human
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REVIEW

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Application Reference
Alzhanova D et al. PLoS Pathog 2021; 17 (1):e1009033 Novel modulators of p53-signaling encoded by unknown genes of emerging viruses.
Application : WB
Reactivity : Human
Sun X et al. Nucleic Acids Res 2020; 48 (16):9109-9123 NRF2 preserves genomic integrity by facilitating ATR activation and G2 cell cycle arrest.
Yenerall P et al. Cell Chem Biol 2020; 27 (1):105-121.e14 RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
Application : WB
Jingwen Li et al. RSC Adv 2019; 9 (67):39082-39089 New spirobisnaphthalenes from an endolichenic fungus strain CGMCC 3.15192 and their anticancer effects through the P53-P21 pathway.
Application : WB
Reactivity : Human
Chen X et al. J Cancer Res Clin Oncol 2019; 145 (6):1471-1484 IL-6 signaling contributes to radioresistance of prostate cancer through key DNA repair-associated molecules ATM, ATR, and BRCA 1/2.
Application : ICC/IF
Reactivity : Human
Burger K et al. Nucleic Acids Res 2019; (Epub) Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks.
Tu HJ et al. Clin Epigenetics 2018; 10 (1):162 The anticancer effects of MPT0G211, a novel HDAC6 inhibitor, combined with chemotherapeutic agents in human acute leukemia cells.
Application : WB
Reactivity : Human
Chen CC et al. Cancers (Basel) 2018; 10 (9) Melatonin Sensitizes Hepatocellular Carcinoma Cells to Chemotherapy Through Long Non-Coding RNA RAD51-AS1-Mediated Suppression of DNA Repair.
Application : WB
Reactivity : Human
Hu L et al. J Immunol 2018; (Epub) TP53 Haploinsufficiency Rescues Emergency Granulopoiesis in FANCC-/- Mice.
Reactivity : Mouse
Furusawa Y et al. Apoptosis 2017; (Epub) Checkpoint kinase 2 is dispensable for regulation of the p53 response but is required for G2/M arrest and cell survival in cells with p53 defects under heat stress.
Application : WB
Reactivity : Human
Glanzer JG et al. Cancer Res 2014; RPA Inhibition increases Replication Stress and Suppresses Tumor Growth.
Application : WB
Gatei M et al. Hum Mol Genet 2014; RAD50 phosphorylation promotes ATR downstream signaling and DNA restart following replication stress.
Application : ICC/IF
Reactivity : Human
Qin B et al. J Biol Chem 2013; 288 (22):16139-44 Ataxia telangiectasia-mutated- and Rad3-related protein regulates the DNA damage-induced G2/M checkpoint through the Aurora A cofactor Bora protein.
Application : WB
Reactivity : Human
Furusawa Y et al. Apoptosis 2012; 17 (1):102-12 Inhibition of checkpoint kinase 1 abrogates G2/M checkpoint activation and promotes apoptosis under heat stress.
Application : WB
Reactivity : Human
Ha K et al. Mol Cancer Ther 2011; 10 (7):1194-206 Hsp90 inhibitor-mediated disruption of chaperone association of ATR with hsp90 sensitizes cancer cells to DNA damage.
Application : ICC/IF
Reactivity : Human
Chen Y et al. Cell Cycle 2011; 10 (4):655-63 Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR.
Application : WB
Reactivity : Human
Zuco V et al. Cancer Lett 2010; 292 (2):186-96 ATM- and ATR-mediated response to DNA damage induced by a novel camptothecin, ST1968.
Application : ICC/IF
Reactivity : Human
Cui B et al. Mol Pharmacol 2009; 75 (6):1356-63 Bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nitrosourea activates the ATR-Chk1 pathway independently of the mismatch repair pathway.
Application : WB
Reactivity : Human
Katsuki Y et al. Cancer Sci 2008; 99 (8):1539-45 Caffeine yields aneuploidy through asymmetrical cell division caused by misalignment of chromosomes.
Application : WB
Reactivity : Human
Kedar PS et al. DNA Repair (Amst) 2008; 7 (11):1787-98 Interaction between PARP-1 and ATR in mouse fibroblasts is blocked by PARP inhibition.
Liu X et al. Mol Cancer Ther 2008; 7 (1):133-42 Ataxia-telangiectasia and Rad3-related and DNA-dependent protein kinase cooperate in G2 checkpoint activation by the DNA strand-breaking nucleoside analogue 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine.
Application : WB
Reactivity : Human
Kulkarni A et al. Am J Physiol Lung Cell Mol Physiol 2008; 294 (5):L998-L1006 Differential roles of ATR and ATM in p53, Chk1, and histone H2AX phosphorylation in response to hyperoxia: ATR-dependent ATM activation.
Application : Functional Assay
Reactivity : Human
Manthey KC et al. J Cell Sci 2007; 120 (Pt 23):847973 NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse.
Application : WB
Reactivity : Human
Wu X et al. Oncogene 2007; 26 (5):757-64 ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation.
Application : ICC/IF
Reactivity : Human
Liu Y et al. J Cell Sci 2006; 119 (Pt 22):1002471 DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A.
Application : ICC/IF
Reactivity : Human
Wu X et al. Cancer Res 2006; 66 (6):2997-3005 Phosphorylation of nucleotide excision repair factor xeroderma pigmentosum group A by ataxia telangiectasia mutated and Rad3-related-dependent checkpoint pathway promotes cell survival in response to UV irradiation.
Application : ICC/IF
Reactivity : Human
Horton JK et al. J Biol Chem 2005; 280 (16):15773-85 Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure.
Application : WB
Li J et al. J Biol Chem 2005; 280 (12):12041-50 Regulation of CHK2 by DNA-dependent protein kinase.
Application : WB
Reactivity : Human
Gibson SL et al. Cancer Res 2005; 65 (23):10734-41 Hypoxia-induced phosphorylation of Chk2 in an ataxia telangiectasia mutated-dependent manner.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations