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ATR antibody [2B5]

Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109
Anti-ATR antibody [2B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70109
Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109
Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

Cat No. GTX70109

Host Mouse
Clonality Monoclonal
Clone Name 2B5
Isotype IgG1
Application WB, ICC/IF, IP, Functional Assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

317 kDa. ( Note )

Positive Control

Raji , Raji nuclear extract
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atr Serine/Threonine Kinase,Fctcs,Frp1,Mec1,Sckl,Sckl1,Atr

Background

Ataxia Telangiectasia Mutated (ATM) and Rad3-related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice.

Database

Research Area

DATA IMAGES
Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

GTX70109 WB Image

Raji whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody (GTX70109) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATR antibody [2B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70109

GTX70109 ICC/IF Image

Immunofluorescence analysis of HeLa, using ATR(GTX70109) antibody at 1:100 dilution.

Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

GTX70109 WB Image

Detection of ATM (Anti-ATM 2C1, GTX70103) or ATR (Anti-ATR 2B5,GTX70109) by western blot in human Raji whole cell extract.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

GTX70109 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] (GTX70109) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
PBS.pdf