ATR antibody

Cat No. GTX10327

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse, Xenopus laevis

See all ATR products

Datasheet
Reference

APPLICATION

Application Note

For WB: Use at a dilution of 0.033 - 0.067 μg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 307 kDa). Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

291 kDa. ( Note )

Positive Control

Whole cell lysate from HeLa and U2OS cells

PROPERTIES

Form

Liquid

Buffer

0.1 ml Tris-citrate/phosphate, pH 7-8, with 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Immunogen was a synthetic peptide, which represented a portion of human ATR and Rad3 related encoded within exon 29 (LocusLink ID 545).

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Atr Serine/Threonine Kinase,Fctcs,Frp1,Mec1,Sckl,Sckl1,Atr

Cellular Localization

Type I membrane protein (Probable).

Background

Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR is a member of the DNA-PK kinases closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes. Several known components of the NuRD complex including histone deacetylase 1 (HDAC1), HDAC2, and CHD4.