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ATR (phospho Thr1989) antibody [GT222]

Orthogonal
Anti-ATR (phospho Thr1989) antibody [GT222] used in Immunoprecipitation (IP). GTX631845
Anti-ATR (phospho Thr1989) antibody [GT222] used in Western Blot (WB). GTX631845
Anti-ATR (phospho Thr1989) antibody [GT222] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631845
Anti-ATR (phospho Thr1989) antibody [GT222] used in Western Blot (WB). GTX631845
Anti-ATR (phospho Thr1989) antibody [GT222] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631845

Cat. No. GTX631845

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT222

Isotype

 IgG2b

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

301 kDa. ( Note )

Positive Control

HCT116 , HCT116(100 J/m2 UVC and recover for 6hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr1989 of human ATR. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888260

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATR (phospho Thr1989) antibody [GT222] used in Immunoprecipitation (IP). GTX631845

GTX631845 IP Image

Immunoprecipitation of ATR protein from HCT-116 whole cell extracts treated with 2 mM Hydroxyurea 6 hrs using 5 μg of ATR antibody (GTX631845) or ATR antibody (GTX128146).
Western blot analysis was performed using ATR antibody (GTX631845) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-ATR (phospho Thr1989) antibody [GT222] used in Western Blot (WB). GTX631845

GTX631845 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Anti-ATR (phospho Thr1989) antibody [GT222] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631845

GTX631845 ICC/IF Image

ATR (phospho Thr1989) antibody [GT222] detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102079) diluted at 1:1000.

Anti-ATR (phospho Thr1989) antibody [GT222] used in Western Blot (WB). GTX631845

GTX631845 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATR (phospho Thr1989) antibody [GT222] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631845

GTX631845 ICC/IF Image

ATR (phospho Thr1989) antibody [GT222] detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169