ATR (phospho Thr1989) antibody [GT222]

ATR (phospho Thr1989) antibody [GT222] detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

ATR (phospho Thr1989) antibody [GT222] detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102079) diluted at 1:1000.

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [GT222] (GTX631845) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Immunoprecipitation of ATR protein from HCT-116 whole cell extracts treated with 2 mM Hydroxyurea 6 hrs using 5 μg of ATR antibody (GTX631845) or ATR antibody (GTX128146).
Western blot analysis was performed using ATR antibody (GTX631845) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.