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ATR (phospho Thr1989) antibody [HL132]

Recombinant antibody Orthogonal
Anti-ATR (phospho Thr1989) antibody [HL132] used in Western Blot (WB). GTX635571
Anti-ATR (phospho Thr1989) antibody [HL132] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635571
Anti-ATR (phospho Thr1989) antibody [HL132] used in IHC (Paraffin sections) (IHC-P). GTX635571
Anti-ATR (phospho Thr1989) antibody [HL132] used in IHC (Paraffin sections) (IHC-P). GTX635571
Anti-ATR (phospho Thr1989) antibody [HL132] used in IHC (Paraffin sections) (IHC-P). GTX635571
Anti-ATR (phospho Thr1989) antibody [HL132] used in IHC (Paraffin sections) (IHC-P). GTX635571

Cat. No. GTX635571

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL132

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ATR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

301 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr1989 of human ATR. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888537

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-ATR (phospho Thr1989) antibody [HL132] used in Western Blot (WB). GTX635571

GTX635571 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATR (phospho Thr1989) antibody [HL132] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635571

GTX635571 ICC/IF Image

Orthogonal

ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ATR (phospho Thr1989) antibody [HL132] used in IHC (Paraffin sections) (IHC-P). GTX635571

GTX635571 IHC-P Image

ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody [HL132] used in IHC (Paraffin sections) (IHC-P). GTX635571

GTX635571 IHC-P Image

ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody [HL132] used in IHC (Paraffin sections) (IHC-P). GTX635571

GTX635571 IHC-P Image

ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody [HL132] used in IHC (Paraffin sections) (IHC-P). GTX635571

GTX635571 IHC-P Image

ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (GTX635571) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations