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ATR (phospho Thr1989) antibody

Anti-ATR (phospho Thr1989) antibody used in Western Blot (WB). GTX128145
Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145
Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145
Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145
Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145
Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145
Anti-ATR (phospho Thr1989) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128145
Anti-ATR (phospho Thr1989) antibody used in Immunoprecipitation (IP). GTX128145
Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145
Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145
Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145
Anti-ATR (phospho Thr1989) antibody used in Western Blot (WB). GTX128145
Anti-ATR (phospho Thr1989) antibody used in Western Blot (WB). GTX128145
Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145
Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145
Anti-ATR (phospho Thr1989) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128145
Anti-ATR (phospho Thr1989) antibody used in Western Blot (WB). GTX128145
Anti-ATR (phospho Thr1989) antibody used in Western Blot (WB). GTX128145

Cat No. GTX128145

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

301 kDa. ( Note )

Positive Control

Raji nuclear extract

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr1989 of human ATR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2687562

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATR (phospho Thr1989) antibody used in Western Blot (WB). GTX128145

GTX128145 WB Image

Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500 and competitor's antibody (CST#2790) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128145

GTX128145 ICC/IF Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Anti-ATR (phospho Thr1989) antibody used in Immunoprecipitation (IP). GTX128145

GTX128145 IP Image

Immunoprecipitation of ATR (phospho Thr1989) protein from HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 μg of ATR (phospho Thr1989) antibody (GTX128145).
Western blot analysis was performed using ATR (phospho Thr1989) antibody (GTX128145).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR protein at nucleus in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human lung by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody used in Western Blot (WB). GTX128145

GTX128145 WB Image

Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATR (phospho Thr1989) antibody used in Western Blot (WB). GTX128145

GTX128145 WB Image

Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody used in IHC (Paraffin sections) (IHC-P). GTX128145

GTX128145 IHC-P Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATR (phospho Thr1989) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128145

GTX128145 ICC/IF Image

ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ATR (phospho Thr1989) antibody used in Western Blot (WB). GTX128145

GTX128145 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:2000.

Anti-ATR (phospho Thr1989) antibody used in Western Blot (WB). GTX128145

GTX128145 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

ATR (phospho Thr1989) antibody Cat No. GTX128145

Rating ( Average 5 based on 16 users reviews)
Application
Western Blot(WB) ( Average 5 based on 13 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2020 in Nat Commun. PMID: 32796829
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019.PMID: 31757956
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018.PMID: 29472538
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Breast Cancer Res in 2017.PMID: 29145865
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2015.PMID: 26684013
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Inflamm Regen in 2019.PMID: 31057688
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal EMBO J in 2018.PMID: 29773570
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018.PMID: 29472538
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017.PMID: 28915641
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017.PMID: 28915641
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2019. PMID: 31135337
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Life Sci Alliance in 2018. PMID: 30456359
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28264028
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 28558031
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2019. PMID: 31135337
Date : Anonymous submitted on 03-Feb-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 cells
Amount used : 20μg
Blocking : 5% milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169