GeneTex
United States (US)

ATRIP antibody [N1], N-term

GTX104654_39638_IFA_19011506_765.jpg
GTX104654_WB_19011506_994.jpg
GTX104654_IHC_19011506_917.jpg

Cat No. GTX104654

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ATRIP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atr Interacting Protein,Atrip

Cellular Localization

Nucleus

Background

The product of this gene is an essential component of the DNA damage checkpoint, and binds to single-stranded DNA coated with replication protein A that accumulates at sites of DNA damage. The encoded protein interacts with the ataxia telangiectasia and Rad3 related protein, a checkpoint kinase, resulting in accumulation of the kinase at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX104654_39638_IFA_19011506_765.jpg

GTX104654 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATRIP(GTX104654) antibody at 1:200 dilution.

GTX104654_WB_19011506_994.jpg

GTX104654 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX104654 diluted at 1:1000

GTX104654_IHC_19011506_917.jpg

GTX104654 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using ATRIP (GTX104654) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169