GeneTex
United States (US)

ATRX antibody [C3], C-term

GTX101310_42165_20161110_WB_shRNA_watermark_19011506_242.jpg
GTX101310_41633_IHC_2_19011506_150.jpg
GTX101310_41787_WB_M_19011506_529.jpg
GTX101310_43005_20180213_IHC-P_19011506_149.jpg
GTX101310_42165_20170223_WB_R_19011506_170.jpg
GTX101310_41633_IHC_3_19011506_928.jpg
GTX101310_41787_20150209_IP_19011506_970.jpg
GTX101310_43075_20171222_WB_19021719_750.jpg
GTX101310_43236_20180905_ICC_IF_19011506_536.jpg

Cat No. GTX101310

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

283 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH3T3 , PC-12 , Rat-2

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATRX. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atrx, Chromatin Remodeler,Jms,Mrx52,Rad54,Rad54L,Xh2,Xnp,Znf-Hx,Atrx

Background

The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101310_42165_20161110_WB_shRNA_watermark_19011506_242.jpg

GTX101310 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (GTX101310) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101310_41633_IHC_2_19011506_150.jpg

GTX101310 IHC-P Image

Rad54 antibody [C3], C-term detects Rad54 protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Rad54 antibody [C3], C-term (GTX101310) dilution: 1:500.

GTX101310_41787_WB_M_19011506_529.jpg

GTX101310 WB Image

ATRX antibody detects ATRX protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody (GTX101310) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101310_43005_20180213_IHC-P_19011506_149.jpg

GTX101310 IHC-P Image

ATRX antibody [C3], C-term detects ATRX protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ATRX stained by ATRX antibody [C3], C-term (GTX101310) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101310_42165_20170223_WB_R_19011506_170.jpg

GTX101310 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (GTX101310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101310_41633_IHC_3_19011506_928.jpg

GTX101310 IHC-P Image

Rad54 antibody [C3], C-term detects Rad54 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Rad54 antibody [C3], C-term (GTX101310) dilution: 1:500.

GTX101310_41787_20150209_IP_19011506_970.jpg

GTX101310 IP Image

Immunoprecipitation of ATRX protein from 293T whole cell extracts using 5 μg of ATRX antibody [C3], C-term (GTX101310).
Western blot analysis was performed using ATRX antibody [C3], C-term (GTX101310).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101310_43075_20171222_WB_19021719_750.jpg

GTX101310 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (GTX101310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101310_43236_20180905_ICC_IF_19011506_536.jpg

GTX101310 ICC/IF Image

ATRX antibody [C3], C-term detects ATRX protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATRX stained by ATRX antibody [C3], C-term (GTX101310) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169