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ATRX antibody [C3], C-term

KO/KD Orthogonal
Anti-ATRX antibody [C3], C-term used in Western Blot (WB). GTX101310
Anti-ATRX antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101310
Anti-ATRX antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101310
Anti-ATRX antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101310
Anti-ATRX antibody [C3], C-term used in Immunoprecipitation (IP). GTX101310
Anti-ATRX antibody [C3], C-term used in Western Blot (WB). GTX101310
Anti-ATRX antibody [C3], C-term used in Western Blot (WB). GTX101310
Anti-ATRX antibody [C3], C-term used in Western Blot (WB). GTX101310
Anti-ATRX antibody [C3], C-term used in Western Blot (WB). GTX101310
Anti-ATRX antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101310
Anti-ATRX antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101310

Cat. No. GTX101310

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ATRX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

283 kDa. ( Note )

Positive Control

NIH3T3 , PC-12 , Rat-2 , 293T

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATRX. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949707

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATRX chromatin remodeler , JMS , MRX52 , RAD54 , RAD54L , XH2 , XNP , ZNF-HX

Background

The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATRX antibody [C3], C-term used in Western Blot (WB). GTX101310

GTX101310 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (GTX101310) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATRX antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101310

GTX101310 IHC-P Image

Rad54 antibody [C3], C-term detects Rad54 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Rad54 antibody [C3], C-term (GTX101310) dilution: 1:500.
Anti-ATRX antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101310

GTX101310 IHC-P Image

ATRX antibody [C3], C-term detects ATRX protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATRX stained by ATRX antibody [C3], C-term (GTX101310) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATRX antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101310

GTX101310 ICC/IF Image

ATRX antibody [C3], C-term detects ATRX protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATRX stained by ATRX antibody [C3], C-term (GTX101310) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-ATRX antibody [C3], C-term used in Immunoprecipitation (IP). GTX101310

GTX101310 IP Image

Immunoprecipitation of ATRX protein from 293T whole cell extracts using 5 μg of ATRX antibody [C3], C-term (GTX101310).
Western blot analysis was performed using ATRX antibody [C3], C-term (GTX101310).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ATRX antibody [C3], C-term used in Western Blot (WB). GTX101310

GTX101310 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (GTX101310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATRX antibody [C3], C-term used in Western Blot (WB). GTX101310

GTX101310 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (GTX101310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATRX antibody [C3], C-term used in Western Blot (WB). GTX101310

GTX101310 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (GTX101310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATRX antibody [C3], C-term used in Western Blot (WB). GTX101310

GTX101310 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (GTX101310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ATRX antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101310

GTX101310 IHC-P Image

ATRX antibody [C3], C-term detects ATRX protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATRX stained by ATRX antibody [C3], C-term (GTX101310) diluted at 1:2500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATRX antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101310

GTX101310 IHC-P Image

ATRX antibody [C3], C-term detects ATRX protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ATRX stained by ATRX antibody [C3], C-term (GTX101310) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Li JN et al. Cancers (Basel) 2021; 13 (24) Expression of SnoRNA U50A Is Associated with Better Prognosis and Prolonged Mitosis in Breast Cancer.
Tanr?kulu B et al. Childs Nerv Syst 2020; (Epub) Is H3K27me3 status really a strong prognostic indicator for pediatric posterior fossa ependymomas? A single surgeon, single center experience.
Application : IHC-P
Reactivity : Human
Raghunandan M et al. Hum Mol Genet 2019; (Epub) Functional crosstalk between the Fanconi anemia and ATRX/DAXX histone chaperone pathways promotes replication fork recovery.
Application : WB
Reactivity : Human
Elmaci ? et al. Clin Neuropathol 2017; (Epub) Paradoxical results obtained with Ki67-labeling and PHH3-mitosis index in glial tumors: a literature analysis.
Reactivity : Human
Yuan Q et al. Int J Nanomedicine 2014; 9 : 4829-46 Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity.
Application : WB
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169