ATRX antibody [GT4212]

Name: ATRX antibody [GT4212]
Brand: GeneTex
SKU: GTX629703
Price: 399.00 USD

Anti-ATRX antibody [GT4212] used in Western Blot (WB). GTX629703

Anti-ATRX antibody [GT4212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629703

Cat. No. GTX629703

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	GT4212
Isotype	IgG1
Application	WB, ICC/IF
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all ATRX products

View product citations for antibody GTX629703 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

283 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , BCL-1 , 293T

Predict Reactivity

Rat, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATRX. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888138

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATRX chromatin remodeler , JMS , MRX52 , RAD54 , RAD54L , XH2 , XNP , ZNF-HX

Cellular Localization

Nucleus

Background

The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Gene ID: 546 ATRX
UniProt: P46100 ATRX

Research Area

DATA IMAGES

GTX629703 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [GT4212] (GTX629703) diluted at 1:500.

GTX629703 ICC/IF Image

ATRX antibody [GT4212] detects ATRX protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATRX protein stained by ATRX antibody [GT4212] (GTX629703) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX629703 WB Image

ATRX antibody [GT4212] detects ATRX protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
5 % SDS-PAGE
ATRX antibody [GT4212] (GTX629703) dilution: 1:500