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ATRX antibody [HL1210]

Recombinant antibody KO/KD Orthogonal
Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478
Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478
Anti-ATRX antibody [HL1210] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635478

Cat. No. GTX635478

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1210

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATRX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

283 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATRX. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888535

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATRX chromatin remodeler , JMS , MRX52 , RAD54 , RAD54L , XH2 , XNP , ZNF-HX

Cellular Localization

Nucleus

Background

The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478

GTX635478 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [HL1210] (GTX635478) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478

GTX635478 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [HL1210] (GTX635478) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ATRX antibody [HL1210] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635478

GTX635478 ICC/IF Image

ATRX antibody [HL1210] detects ATRX protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATRX stained by ATRX antibody [HL1210] (GTX635478) diluted at 1:100.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations