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ATXN2 antibody [HL1902]

Recombinant antibody
Anti-ATXN2 antibody [HL1902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637651
Anti-ATXN2 antibody [HL1902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637651
Anti-ATXN2 antibody [HL1902] used in IHC (Paraffin sections) (IHC-P). GTX637651
Anti-ATXN2 antibody [HL1902] used in Western Blot (WB). GTX637651

Cat. No. GTX637651

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1902

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human ATXN2

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ataxin 2 , ATX2 , SCA2 , TNRC13

Cellular Localization

Cytoplasm

Background

This gene belongs to a group of genes that is associated with microsatellite-expansion diseases, a class of neurological and neuromuscular disorders caused by expansion of short stretches of repetitive DNA. The protein encoded by this gene has two globular domains near the N-terminus, one of which contains a clathrin-mediated trans-Golgi signal and an endoplasmic reticulum exit signal. The encoded cytoplasmic protein localizes to the endoplasmic reticulum and plasma membrane, is involved in endocytosis, and modulates mTOR signals, modifying ribosomal translation and mitochondrial function. The N-terminal region of the protein contains a polyglutamine tract of 14-31 residues that can be expanded in the pathogenic state to 32-200 residues. Intermediate length expansions of this tract increase susceptibility to amyotrophic lateral sclerosis, while long expansions of this tract result in spinocerebellar ataxia-2, an autosomal-dominantly inherited, neurodegenerative disorder. Genome-wide association studies indicate that loss-of-function mutations in this gene may be associated with susceptibility to type I diabetes, obesity and hypertension. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]

Database

Research Area

DATA IMAGES

Anti-ATXN2 antibody [HL1902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637651

GTX637651 ICC/IF Image

ATXN2 antibody [HL1902] detects ATXN2 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATXN2 stained by ATXN2 antibody [HL1902] (GTX637651) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ATXN2 antibody [HL1902] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637651

GTX637651 ICC/IF Image

ATXN2 antibody [HL1902] detects ATXN2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATXN2 stained by ATXN2 antibody [HL1902] (GTX637651) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ATXN2 antibody [HL1902] used in IHC (Paraffin sections) (IHC-P). GTX637651

GTX637651 IHC-P Image

ATXN2 antibody [HL1902] detects ATXN2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ATXN2 stained by ATXN2 antibody [HL1902] (GTX637651) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATXN2 antibody [HL1902] used in Western Blot (WB). GTX637651

GTX637651 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATXN2 antibody [HL1902] (GTX637651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations