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AUF1 (phospho Ser83) antibody

Anti-AUF1 (phospho Ser83) antibody used in Western Blot (WB). GTX55319
Anti-AUF1 (phospho Ser83) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55319
Anti-AUF1 (phospho Ser83) antibody used in IHC (Paraffin sections) (IHC-P). GTX55319

Cat. No. GTX55319

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of Serine 83(N-S-S(p)-P-R) derived from human AUF1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein D , AUF1 , AUF1A , HNRPD , P37 , hnRNPD0

Cellular Localization

Nucleus,Cytoplasm

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AUF1 (phospho Ser83) antibody used in Western Blot (WB). GTX55319

GTX55319 WB Image

WB analysis of extracts from JK cells (lane 2) using GTX55319 AUF1 (phospho Ser83) antibody.
Left : Primary antibody pre-incubated with the antigen specific peptide
Right : Primary antibody

Anti-AUF1 (phospho Ser83) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55319

GTX55319 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX55319 AUF1 (phospho Ser83) antibody.

Anti-AUF1 (phospho Ser83) antibody used in IHC (Paraffin sections) (IHC-P). GTX55319

GTX55319 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX55319 AUF1 (phospho Ser83) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399