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AVEN antibody [3G4]

Anti-AVEN antibody [3G4] used in Western Blot (WB). GTX50039
Anti-AVEN antibody [3G4] used in IHC (Paraffin sections) (IHC-P). GTX50039

Cat No. GTX50039

Host

Mouse

Clonality

Monoclonal

Clone Name

3G4

Isotype

IgG2a

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000~1:2,000
Immunohistochemistry: Use at 1:50~100.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human AVEN (254-362aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Apoptosis And Caspase Activation Inhibitor , Pdcd12 , Aven

Cellular Localization

Associated with intracellular membranes.

Background

PDCD12 (Programmed cell death 12), also referred to as AVEN, represents a new class of cell death regulator. PDCD12 is a conserved intracellular membrane protein that blocks apoptosis impeded by Apaf-1 mediated caspase activation. Bcl-2 and BAD also interact with PDCD12 to prevent apoptosis. It is expressed in a wide variety of normal tissues including heart, skeletal muscle, kidney, liver, testis, and diseases such as cancer

Database

Research Area

DATA IMAGES

Anti-AVEN antibody [3G4] used in Western Blot (WB). GTX50039

GTX50039 WB Image

The extracts of mouse kidney (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-AVEN antibody [3G4] used in IHC (Paraffin sections) (IHC-P). GTX50039

GTX50039 IHC-P Image

Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf