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AXL antibody [C2C3], C-term

KO/KD Overexpression
Anti-AXL antibody [C2C3], C-term used in Western Blot (WB). GTX101345
Anti-AXL antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101345
Anti-AXL antibody [C2C3], C-term used in Western Blot (WB). GTX101345
Anti-AXL antibody [C2C3], C-term used in Western Blot (WB). GTX101345

Cat. No. GTX101345

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AXL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

H1299 , AXL transfected 293T , HeLa

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human AXL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949710

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AXL receptor tyrosine kinase , ARK , JTK11 , Tyro7 , UFO

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AXL antibody [C2C3], C-term used in Western Blot (WB). GTX101345

GTX101345 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AXL antibody [C2C3], C-term (GTX101345) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AXL antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101345

GTX101345 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using AXL(GTX101345) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AXL antibody [C2C3], C-term used in Western Blot (WB). GTX101345

GTX101345 WB Image

Overexpression

Western Blot analysis of AXL expression in transfected 293T cell line by AXL polyclonal antibody.
A: Non-transfected lysate.
B: AXL transfected lysate.
7.5% SDS PAGE
GTX101345 diluted at 1:500

Anti-AXL antibody [C2C3], C-term used in Western Blot (WB). GTX101345

GTX101345 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
GTX101345 diluted at 1:500

REFERENCE

Application Reference

Tsai LH et al. Oncogene 2014; The MZF1/c-MYC axis mediates lung adenocarcinoma progression caused by wild-type lkb1 loss.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169