GeneTex
United States (US)

AXL antibody [C2C3], C-term

GTX101345_39812_20180907_WB_shRNA_watermark_19011506_505.jpg
GTX101345_39812_WB_19011506_838.jpg
GTX101345_WB_19011506_259.jpg
GTX101345_39812_IHC_19011506_506.jpg

Cat No. GTX101345

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

H1299 , AXL tansfected 293T cell

Predict Reactivity

Mouse, Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human AXL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Axl Receptor Tyrosine Kinase,Ark,Jtk11,Tyro7,Ufo,Axl

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101345_39812_20180907_WB_shRNA_watermark_19011506_505.jpg

GTX101345 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AXL antibody [C2C3], C-term (GTX101345) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101345_39812_WB_19011506_838.jpg

GTX101345 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
GTX101345 diluted at 1:500

GTX101345_WB_19011506_259.jpg

GTX101345 WB Image

Western Blot analysis of AXL expression in transfected 293T cell line by AXL polyclonal antibody.
A: Non-transfected lysate.
B: AXL transfected lysate.
7.5% SDS PAGE
GTX101345 diluted at 1:500

GTX101345_39812_IHC_19011506_506.jpg

GTX101345 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using AXL(GTX101345) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169