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AXL antibody

KO/KD Overexpression
Anti-AXL antibody used in Western Blot (WB). GTX108560
Anti-AXL antibody used in IHC (Paraffin sections) (IHC-P). GTX108560
Anti-AXL antibody used in Western Blot (WB). GTX108560
Anti-AXL antibody used in Western Blot (WB). GTX108560

Cat. No. GTX108560

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AXL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

A549 , H1299 , human AXL-transfected 293T cells , HeLa

Predict Reactivity

Mouse, Rat, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human AXL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949711

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AXL receptor tyrosine kinase , ARK , JTK11 , Tyro7 , UFO

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AXL antibody used in Western Blot (WB). GTX108560

GTX108560 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX108560) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AXL antibody used in IHC (Paraffin sections) (IHC-P). GTX108560

GTX108560 IHC-P Image

AXL antibody detects AXL protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
AXL stained by AXL antibody (GTX108560) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AXL antibody used in Western Blot (WB). GTX108560

GTX108560 WB Image

Sample (30 μg of whole cell lysate)
A: A549
B: H1299
5% SDS PAGE
GTX108560 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AXL antibody used in Western Blot (WB). GTX108560

GTX108560 WB Image

Overexpression

AXL antibody detects AXL protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human AXL-transfected 293T cells
7.5% SDS-PAGE
AXL antibody (GTX108560) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lee PC et al. Cancer Cell 2018; 34 (6) : 954-969.e4 Targeting PKCδ as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169