Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX108560) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

AXL antibody detects AXL protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human AXL-transfected 293T cells
7.5% SDS-PAGE
AXL antibody (GTX108560) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: A549
B: H1299
5% SDS PAGE
GTX108560 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.