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AXL antibody

KO/KD Overexpression
Anti-AXL antibody used in IHC (Paraffin sections) (IHC-P). GTX129407
Anti-AXL antibody used in IHC (Paraffin sections) (IHC-P). GTX129407
Anti-AXL antibody used in Western Blot (WB). GTX129407
Anti-AXL antibody used in IHC (Paraffin sections) (IHC-P). GTX129407
Anti-AXL antibody used in Western Blot (WB). GTX129407
Anti-AXL antibody used in IHC (Paraffin sections) (IHC-P). GTX129407
Anti-AXL antibody used in ELISA (ELISA). GTX129407
Anti-AXL antibody used in Western Blot (WB). GTX129407

Cat. No. GTX129407

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AXL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA 1:-1:10000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

human AXL-transfected 293T , HeLa

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AXL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885984

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AXL receptor tyrosine kinase , ARK , JTK11 , Tyro7 , UFO

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AXL antibody used in IHC (Paraffin sections) (IHC-P). GTX129407

GTX129407 IHC-P Image

AXL antibody detects AXL protein at cell membrane in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: AXL antibody (GTX129407) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AXL antibody used in IHC (Paraffin sections) (IHC-P). GTX129407

GTX129407 IHC-P Image

AXL antibody detects AXL protein at membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
AXL antibody (GTX129407) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AXL antibody used in Western Blot (WB). GTX129407

GTX129407 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX129407) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AXL antibody used in IHC (Paraffin sections) (IHC-P). GTX129407

GTX129407 IHC-P Image

AXL antibody detects AXL protein at membrane on human non-small cell lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human non-small cell lung carcinoma.
AXL antibody (GTX129407) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AXL antibody used in Western Blot (WB). GTX129407

GTX129407 WB Image

Overexpression

AXL antibody detects AXL protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human AXL-transfected 293T cells
7.5 % SDS-PAGE
AXL antibody (GTX129407) dilution: 1:5000

Anti-AXL antibody used in IHC (Paraffin sections) (IHC-P). GTX129407

GTX129407 IHC-P Image

AXL antibody detects AXL protein at cell membrane on human non-small cell lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human non-small cell lung carcinoma.
AXL antibody (GTX129407) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AXL antibody used in ELISA (ELISA). GTX129407

GTX129407 ELISA Image

An ELISA plate is coated with 50 μL of AXL recombinant protein at concentration of 4 μg/mL. The coated protein is detected with anti-AXL antibody (GTX129407) at concentration ranged from 0.25 to 1000 ng/mL.

Anti-AXL antibody used in Western Blot (WB). GTX129407

GTX129407 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX129407) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Tsugawa H et al. PLoS Pathog 2023; 19 (6) : e1011139 Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae, to the liver.
Trinh QD et al. Cells 2022; 11 (19) TGF-β1 Promotes Zika Virus Infection in Immortalized Human First-Trimester Trophoblasts via the Smad Pathway.
Huang C et al. J Agric Food Chem 2019; 67 (10) : 2818-2830 Ampelopsins A and C Induce Apoptosis and Metastasis through Downregulating AxL, TYRO3, and FYN Expressions in MDA-MB-231 Breast Cancer Cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169