GeneTex
United States (US)

AXL antibody

GTX129407_41619_IHC_2_18121410_575.jpg
GTX129407_41619_20180907_WB_shRNA_watermark_18121410_392.jpg
GTX129407_41619_20150408_IHC-P_18121410_896.jpg
GTX129407_41619_WB_B_18121410_766.jpg
GTX129407_41619_20141119_IHC_18121410_574.jpg
GTX129407_41619_20151217_ELISA_18121410_107.jpg
GTX129407_41619_20141017_IHC_3_18121410_708.jpg
GTX129407_43222_20180601_WB_18121410_474.jpg

Cat No. GTX129407

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA 1:-1:10000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

H1299 , human AXL-transfected 293T

Predict Reactivity

Mouse, Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AXL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Axl Receptor Tyrosine Kinase,Ark,Jtk11,Tyro7,Ufo,Axl

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX129407_41619_IHC_2_18121410_575.jpg

GTX129407 IHC-P Image

AXL antibody detects AXL protein at membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
AXL antibody (GTX129407) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX129407_41619_20180907_WB_shRNA_watermark_18121410_392.jpg

GTX129407 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX129407) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129407_41619_20150408_IHC-P_18121410_896.jpg

GTX129407 IHC-P Image

AXL antibody detects AXL protein at cell membrane in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: AXL antibody (GTX129407) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX129407_41619_WB_B_18121410_766.jpg

GTX129407 WB Image

AXL antibody detects AXL protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human AXL-transfected 293T cells
7.5 % SDS-PAGE
AXL antibody (GTX129407) dilution: 1:5000

GTX129407_41619_20141119_IHC_18121410_574.jpg

GTX129407 IHC-P Image

AXL antibody detects AXL protein at cell membrane on human non-small cell lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human non-small cell lung carcinoma.
AXL antibody (GTX129407) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX129407_41619_20151217_ELISA_18121410_107.jpg

GTX129407 ELISA Image

An ELISA plate is coated with 50 μL of AXL recombinant protein at concentration of 4 μg/mL. The coated protein is detected with anti-AXL antibody (GTX129407) at concentration ranged from 0.25 to 1000 ng/mL.

GTX129407_41619_20141017_IHC_3_18121410_708.jpg

GTX129407 IHC-P Image

AXL antibody detects AXL protein at membrane on human non-small cell lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human non-small cell lung carcinoma.
AXL antibody (GTX129407) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX129407_43222_20180601_WB_18121410_474.jpg

GTX129407 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX129407) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169