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Acetyl-CoA Carboxylase 1 antibody

Anti-Acetyl-CoA Carboxylase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132081
Anti-Acetyl-CoA Carboxylase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132081
Anti-Acetyl-CoA Carboxylase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132081
Anti-Acetyl-CoA Carboxylase 1 antibody used in Western Blot (WB). GTX132081

Cat No. GTX132081

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

266 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Acetyl CoA Carboxylase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

acetyl-CoA carboxylase alpha,ACAC,ACACAD,ACC,ACC1,ACCA

Cellular Localization

Cytoplasm

Background

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 31 ACACA
  • UniProt: Q13085 ACACA

Research Area

DATA IMAGES
Anti-Acetyl-CoA Carboxylase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132081

GTX132081 IHC-P Image

Acetyl CoA Carboxylase 1 antibody detects Acetyl CoA Carboxylase 1 protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Acetyl CoA Carboxylase 1 antibody (GTX132081) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Acetyl-CoA Carboxylase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132081

GTX132081 ICC/IF Image

Acetyl CoA Carboxylase 1 antibody detects Acetyl CoA Carboxylase 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Acetyl CoA Carboxylase 1 protein stained by Acetyl CoA Carboxylase 1 antibody (GTX132081) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Acetyl-CoA Carboxylase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132081

GTX132081 IHC-P Image

Acetyl CoA Carboxylase 1 antibody detects Acetyl CoA Carboxylase 1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Acetyl CoA Carboxylase 1 antibody (GTX132081) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Acetyl-CoA Carboxylase 1 antibody used in Western Blot (WB). GTX132081

GTX132081 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl CoA Carboxylase 1 antibody (GTX132081) diluted at 1:1000.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169