Acetyl-CoA Carboxylase 1 antibody

Cat. No. GTX132081

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX132081 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

266 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Acetyl-CoA Carboxylase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886558

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acetyl-CoA carboxylase alpha , ACAC , ACACAD , ACC , ACC1 , ACCA

Cellular Localization

Cytoplasm

Background

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 31 ACACA
UniProt: Q13085 ACACA

Research Area

DATA IMAGES

GTX132081 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl-CoA Carboxylase 1 antibody (GTX132081) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132081 WB Image

GTX132081 ICC/IF Image

Acetyl CoA Carboxylase 1 antibody detects Acetyl CoA Carboxylase 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Acetyl CoA Carboxylase 1 protein stained by Acetyl CoA Carboxylase 1 antibody (GTX132081) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX132081 IHC-P Image

Acetyl CoA Carboxylase 1 antibody detects Acetyl CoA Carboxylase 1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Acetyl CoA Carboxylase 1 antibody (GTX132081) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX132081 IHC-P Image

Acetyl CoA Carboxylase 1 antibody detects Acetyl CoA Carboxylase 1 protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Acetyl CoA Carboxylase 1 antibody (GTX132081) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min