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Aconitase 1 antibody [N1C1]

Anti-Aconitase 1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102974
Anti-Aconitase 1 antibody [N1C1] used in Western Blot (WB). GTX102974

Cat No. GTX102974

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aconitase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aconitase 1 , ACONS , HEL60 , IREB1 , IREBP , IREBP1 , IRP1

Cellular Localization

Cytoplasm

Background

Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Aconitase 1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102974

GTX102974 ICC/IF Image

Aconitase 1 antibody [N1C1] detects Aconitase 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Aconitase 1 protein stained by Aconitase 1 antibody [N1C1] (GTX102974) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Aconitase 1 antibody [N1C1] used in Western Blot (WB). GTX102974

GTX102974 WB Image

Aconitase 1 antibody detects Aconitase 1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Aconitase 1 antibody (GTX102974) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169