*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , HeLa , HepG2 , HCT116
Mouse, Rat, Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.78 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human Aconitase 1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes. [provided by RefSeq]
GTX102974 ICC/IF Image
Aconitase 1 antibody [N1C1] detects Aconitase 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Aconitase 1 protein stained by Aconitase 1 antibody [N1C1] (GTX102974) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX102974 WB Image
Aconitase 1 antibody detects Aconitase 1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Aconitase 1 antibody (GTX102974) dilution: 1:1000