GeneTex
United States (US)

Aconitase 1 antibody

GTX64472_20180530_IHC-P_1_18121411_626.jpg
GTX64472_20180530_WB_18121411_518.jpg
GTX64472_20180530_IHC-P_18121411_296.jpg

Cat No. GTX64472

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

98 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-170 of human ACO1 (NP_002188.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IREBP1 Antibody , IREB1 Antibody , ACONS Antibody , ACO1 Antibody , IRP1 Antibody , IREBP Antibody

Background

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES
GTX64472_20180530_IHC-P_1_18121411_626.jpg

GTX64472 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX64472 Aconitase 1 antibody.
Dilution : 1:100

GTX64472_20180530_WB_18121411_518.jpg

GTX64472 WB Image

WB analysis of various samples using GTX64472 Aconitase 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX64472_20180530_IHC-P_18121411_296.jpg

GTX64472 IHC-P Image

IHC-P analysis of human prostate tissue using GTX64472 Aconitase 1 antibody.
Dilution : 1:100

Package List Price ($)
$ 319