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Aconitase 2 antibody [3G8]

Overexpression
Anti-Aconitase 2 antibody [3G8] used in Western Blot (WB). GTX84963
Anti-Aconitase 2 antibody [3G8] used in Western Blot (WB). GTX84963
Anti-Aconitase 2 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84963
Anti-Aconitase 2 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84963
Anti-Aconitase 2 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84963
Anti-Aconitase 2 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84963
Anti-Aconitase 2 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84963

Cat. No. GTX84963

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all Aconitase 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ACO2 (NP_001089) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aconitase 2 , ACONM , HEL-S-284 , ICRD , OCA8 , OPA9

Cellular Localization

Mitochondrion

Background

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

Database

Research Area

DATA IMAGES

Anti-Aconitase 2 antibody [3G8] used in Western Blot (WB). GTX84963

GTX84963 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84963 Aconitase 2 antibody [3G8].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Aconitase 2 antibody [3G8] used in Western Blot (WB). GTX84963

GTX84963 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Aconitase 2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84963 Aconitase 2 antibody [3G8].
Loading : 5 ug per lane

Anti-Aconitase 2 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84963

GTX84963 IHC-P Image

IHC-P analysis of kidney tissue using GTX84963 Aconitase 2 antibody [3G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Aconitase 2 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84963

GTX84963 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Aconitase 2 plasmid using GTX84963 Aconitase 2 antibody [3G8].

Anti-Aconitase 2 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84963

GTX84963 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84963 Aconitase 2 antibody [3G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Aconitase 2 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84963

GTX84963 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84963 Aconitase 2 antibody [3G8].

Anti-Aconitase 2 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84963

GTX84963 IHC-P Image

IHC-P analysis of ovary tissue using GTX84963 Aconitase 2 antibody [3G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519