APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.01-0.03μg/ml |
| 3-5μg/ml |
|
Note :
IHC-P
Human Breast shows mitochondrial staining in the epithelial cells of the ducts.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88183-PEP).
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS pH7.3, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QDTYQHPPKDSSGQH, from the internal region of the protein sequence according to NP_001089.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aconitase 2 , ACONM , HEL-S-284 , ICRD , OCA8 , OPA9
Cellular Localization
Mitochondrion
Background
The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX88183 IHC-P Image
IHC-P analysis of human breast using GTX88183 Aconitase 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
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GTX88183 WB Image
WB analysis of human (A), mouse (B) and rat (C) adipose lysates using GTX88183 Aconitase 2 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88183 WB Image
WB analysis of human heart lysate using GTX88183 Aconitase 2 antibody, Internal.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88183 WB Image
WB analysis of mouse (A), rat (B) and pig (C) skeletal muscle lysates using GTX88183 Aconitase 2 antibody, Internal.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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